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دکتر مسعود پارسانیا  PhD

 

مدیر کیفی آزمایشگاه

 

 

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داخلی 109

 

سوابق تحصیلی

 

    Tarbiat Modarres University, Tehran / Iran
Sep 23. 2002 – May 25, 2008: PhD. in Virology.
The effect of apoptosis ( BAX ) and antiapoptosis (Bcl-XL ) genes on efficiency of DNA vaccine encoding glycoprotein B of herpes simplex virus type 1.
Supervisor: Dr. Zuhair Mohammad Hassan
Advisors: 1- Dr. Taravat Bamdad 2- Dr. M.aryam Kheirandish


    Iran Medical Sciences University, Tehran / Iran

1994 – 1997: Master of Science in Virology.

 

    Tehran Medical Sciences Branch, Islamic Azad University, Tehran / Iran 

1985 – 1990: BSc in Laboratory Technology.

 

سوابق اجرایی

  Teaching:                                                                                                                                                                             
 2005-Present: Teaching Virology for Medical students of Tehran Medical Sciences Branch, Islamic Azad University.
 2006-Present: Teaching Virology for Biology students of Tehran Medical Sciences Branch, Islamic Azad University.
 1997-Present: Teaching Virology for Laboratory Technology students of Tehran Medical Sciences Branch, Islamic Azad University.
 2012-Present: Teaching Cell Culture for Genetics students (MS Student) of Tehran Medical Sciences Branch, Islamic Azad University.                                                                                                                                                                                                                                                                                                                                    

 

سوابق پژوهشی

                                                                                                                                                                                                                                                                      :Research

 2012-2014: Executer of research project: Evaluation of Human Metapneumovirus and Respiratory Syncytial Virus Infections Prevalence by Molecular Method in Children with Lower Respiratory Tract Infection hospitalized to Tehran Ali Asghar Hospital During 2010 to 2012
 2011-2013: Executer of research project: Evaluation of anti-viral effects of five medicinal plant species against of Herpes simplex virus Type 1 on Human cell culture
 2005-2008: Thesis Research Project: The effect of apoptosis ( BAX ) and antiapoptosis (Bcl-XL ) genes on efficiency of DNA vaccine encoding glycoprotein B of herpes simplex virus type 1.Tarbiat Modarres University, Tehran, Iran.
 2006-2009: Research Assistant, The effect of supernatant of necrotic cells as an innate adjuvant on efficiency of DNA vaccine encoding glycoprotein B of Herpes Simplex virus type1. Tarbiat Modarres University, Tehran, Iran.
 2006-2009: Research Assistant, Evaluation the Immunological Effects of DNA Vaccine Encoding VP22 of Herpes Simplex Virus Type 1. Tarbiat Modarres University, Tehran, Iran.
 2007-2009: Research Assistant, Enhancement of a DNA vaccine immunogenicity against herpes simplex virus type 1 by co-administration of LIGHT expression plasmid as a genetic adjuvant. Tarbiat Modarres University, Tehran, Iran.
 2007-2009: Research Assistant, Kinetics of primary and memory cytotoxic T lymphocyte responses to herpes simplex virus 1 infection: granzyme B mediated CTL activity. Tarbiat Modarres University, Tehran, Iran.

 

Experiences:

 2005-2007: Research Assistant, Evaluation of g-interferon kinetics in HSV-1 infected mice in different days post infection (in vivo) and post re-stimulation (in vitro). Tarbiat Modarres University, Tehran, Iran.
 2005-2007: Research Assistant, Naloxone, an opioid receptor antagonist, enhances induction of protective immunity against HSV-1 infection in BALB/c mice. Tarbiat Modarres University, Tehran, Iran.
 2004-2005: Research Assistant, An efficient method for immortalization of genetics disorders patients lymphocytes by Epstein-Barr virus (EBV). Research Center of (IBTO) Iranian Blood Transfusion organization, Tehran, Iran
 2002-2003: Research Assistant, Validation of Viral Inactivation Method of Pasteurization on The Albumin 20% Product Manufactured By I.B.R.F.Co . Iranian Blood Research and Fractionation Co, Tehran, Iran.
 2001-2002: Research Assistant, Evaluation of Anti HBs Antibody levels in serum of workers in Amir- Al-Moemenin General Hospital, Tehran, Iran.
Supervisor of Thesis:
 2017: Study of Prevalence of High-risk human papillomavirus (HPV) genotypes in Married Women resident in Kermanshah using PCR-Hybridization method from April to December 2017.
 2017: Evaluation of anti-cancer effect of Chelidonium majus L. alkaloidal extract on MCF-7 breast cancer cell line and Hela cervical cancer cell line
 2016: Quantitative detection of Human Papilloma virus E6 oncogen mRNA by Real time PCR in Tc-1 cell line
 2016: Quantitative detection of Influenza H1N1 haemagglutinin by Real time PCR to evaluate candidate drugs for influenza
 2015: Evaluation and comparison of antiviral effects of hexane, chloroform, methanol and Aqueous extracts of Chelidonium majus against Herpes Simplex Virus type 1 in Hela cell culture
 2015: Evaluation and comparing antiviral effects activity of two methanolic and chlroformic extracts of Chelidonium Majus against Herpes simplex virus type 1(HSV1) by quantitative Real-time PCR in human Hela cell culture
 2015: Evaluation and comparison of antiviral effects of Chelidonium majus hexane and aqueous extracts against Herpes simplex virus type 1 in Hela cell culture by quantitative real-time PCR
 2015: Evaluation of Chelidonium majus L. extract effects against Herpes simplex virus type 1 compared with Acyclovir
 2014: Evaluation antiviral effects of extracts of Rosmarinus officinalis against herpes simplex virus type 1 in cell culture.
 2013: Evaluation Antiviral effects of extracts of Satureja against herpes simplex virus type1 in cell culture
 2013: Evaluation Antiviral effects of extracts of Aloe vera against herpes simplex virus type1 in cell culture
 2012: Evaluation of anti-viral effects of Ruta graveolens L.extract against of Herpes simplex virus Type 1 on Human cell culture.
 2012: Evaluation of anti-viral effects of Thymus vulgaris L. extract against of Herpes simplex virus Type 1 on Human cell culture
 2011: Follow up of patients with lower respiratory tract infection due to respiratory syncitial virus discharged from Ali-Asghar Hospital from 2009 to 2011
 2011: 6-month to 3-year follow up of patients with upper respiratory tract infection due to respiratory syncitial virus attending to Ali-Asghar Hospital from 2009 to 2011
 2011: Evaluation of prevalence of HCV in hemodialysis patients of Rasht’s Razi hospital based on serologic and molecular assessment in 1391

                                                                                                                                                                                                                                           

مقالات و انتشارات

                                                                                                                                                                                                                                                                   :Publications

1. Masoud Parsania, Mohammad-Bagher Rezaee, Seyed Hamidreza Monavari, Kamkar Jaimand , Seyed Milad Mousavi-Jazayeri, Mehdi Razazian, Mohammad Hossein Nadjarha Antiviral screening of four plant extracts against acyclovir resistant herpes simplex virus type-1. July 2017 Pak. J. Pharm. Sci., Vol.30, No.4(Suppl), , pp.1407-1411
2. M Sadeghpour Natanzi, M Parsania, M Aminzadeh. Antiviral and virucidal activity of Chelidonium majus L. extract compared with Acyclovir against Herpes simplex virus type 1. 2017 Armaghane danesh 21 (11), 1056-1068
3. Seyed Mohamad javad Taher Mofrad, Mohsen Lotfi, Masoud Parsania. Comparision of Vero and New Suspension Cell Line in Propagation of Peste des Petits Ruminants Virus (PPRV). 2016 Sep Journal of Advanced Agricultural Technologies
4. Parsania M, Poopak B, Pouriayevali MH, Haghighi S, Amirkhani A, Nateghian A. Detection of Human Metapneumovirus and Respiratory Syncytial Virus by Real-Time Polymerase Chain Reaction Among Hospitalized Young Children in Iran. 2016 Mar Jundishapur J Microbiol.;9(3):e32974.
5. G Yousefi Kordestani, M Parsania. Evaluation antiviral effect of leaf extract of Rosmarinus officinalis against Herpes simplex virus type 1 in cell culture. 2015 Armaghane danesh 20 (8), 706-719
6. Alireza Nateghian, Sama Haghighi, Behnaz Parvini, Behzad Poopak, Masoud Parsania. Comparison of clinical. demographic and laboratory data from lower respiratory tract infection due to respiratory syncytialvirus and human metapneumovirus. 2015 Razi Journal of Medical Sciences 22 (134), 84-90
7. Dorostkar R, Bamdad T, Parsania M, Pouriayevali H. An endogenous immune adjuvant released by necrotic cells for enhancement of DNA vaccine potency. Iran J Immunol. 2012 Dec;9(4):215-25
8. Gorgian Mohammady M, Bamdad T, Parsania M, Soleimanjahi H, Pouyanfard S, Hashemi H, Asghari-Jafarabadi M. Effect of LIGHT adjuvant on kinetics of T-cell responses induced by HSV-1 DNA immunization. Iran J Immunol. 2011 Jun;8(2):76-84.
9. Pouriayevali MH, Bamdad T, Parsania M, Sari RD. Full length antigen priming enhances the CTL epitope-based DNA vaccine efficacy. Cell Immunol. 2011;268(1):4-8.
10. Parsania M, Bamdad T, Hassan ZM, Kheirandish M, Pouriayevali MH, Dorostkar Sari R, Jamali A. Evaluation of apoptotic and anti-apoptotic genes on efficacy of DNA vaccine encoding glycoprotein B of Herpes Simplex Virus type 1. Immunology Letters. 2010;128(2):137-142.
11. Pouyanfard S, Bamdad T, Parasania M,Hashemi H, Gorgian Mohammadi M.Impact of timing strategy of LIGHT, a new TNF superfamily on immune platform induced by HSV-1 gB DNA vaccine. Cytokine. 2010;50(1):99-103.
12. Arefian E, Bamdad T, Soleimanjahi H, Akhoond M.R, Parsania M, Ghaemi A. A Kinetic Study of Gamma Interferon Production in Herpes Simplex Virus–1 DNA Prime-Protein Boost Regimen Comparing to DNA or Subunit Vaccination 1. MOLECULAR BIOLOGY. 2009;43(3):388-393.
13. Gorgian Mohammadi M, Bamdad T, Parsania M, Hashemi H.R, Puyanfard S. Kinetics of primary and memory cytotoxic T lymphocyte responses to herpes simplex virus 1 infection: granzyme B mediated CTL activity. Iran J Immunol. 2009 Mar;6(1):22-7. 2009;6(1):22-27
14. Parsania M, Hassan ZM, Bamdad T, Kheirandish M, Sarbolouki M.N, Pouriayevali MH, Dorostkar Sari R, Mahdavi M, Jamali A. Evaluation of Bax Encoding Plasmid on Increase Efficacy of DNA Vaccine Plasmid Encoding gB of Herpes Simplex Virus Type 1. Yakhteh Medical Journal. 2008;2:93-100
15. Parsania M, Hassan ZM, Bamdad T, Kheirandish M, Sarbolouki M.N, Pouriayevali MH, Dorostkar Sari R, Jamali A, Mahdavi M. Pro-apoptotic gene enhances the immunogenicity of glycoprotein B gene of herpes simplex virus-1. Gene Therapy and Molecular Biology. 2008;12:7-14.
16. Arefian E, Bamdad T, Soleimanjahi H, Akhoond M.R, Parsania M, Ghaemi A. Evaluation of g-interferon kinetics in HSV-1 infected mice in different days post infection (in vivo) and post re-stimulation (in vitro). Comparative Immunology, Microbiology & Infectious Diseases. 2007;30:1-9.
17. Sabahi F, Javan M, Jazayeri Farsani M, Parsania M, Bamdad T. Naloxone, an opioid receptor antagonist, enhances induction of protective immunity against HSV-1 infection in BALB/c mice. Microbial Pathogenesis. 2007;43:217-223.
18. Zangeneh M, Poopak B, Khavari Z, Valikhani M, Parsania M. Evaluation of Immunogenicity of Hepatitis B Vaccination In Health workers. Medical Sciences Journal of Islamic Azad University-Tehran Medical Branch, 2004;37:13-21.                                                                                                                                                                                                                                                 

 

مهارت ها 

      Skills                                                                                                                                                                                                                                                                 
 Cell culture technique and virus isolation and virus titration (by TCID50 assay).
 ELISA technique (Quantitative & Qualitative}.
 Transformation of bacteria and plasmid extraction (minipreparation & Maxipreparation) .
 CTL assay by Flow Cytometry
 Molecular Techniques, DNA extraction, PCR techniques, agarose & PAGE Electrophoresis.                                                                                                                                                                                                                                                      

 

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