Flow Cytometry

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Flowcytometry is a very fast and powerful method used to identify cells and classify them. In this method, the cells in the suspension are passed through a laminar flow from the flow cell and in front of the laser light, and in terms of physical characteristics such as size, granularity, shape and complexity of the nucleus, and also in terms of indicators on the surface of the membrane, the cytoplasm and nucleus by monoclonal antibodies that are labeled with fluorescence materials are investigated.

In the Flowcytometry section of Payvand Laboratory, by using the latest modern flowcytometers (BD Biosciences FACSLyric 10-color), in addition to counting, determining the volume and granularity of cells, it can be used to study surface markers (CD markers) at the surface, cytoplasm or cell nucleus, and to use the results in order to diagnose, categorize, prognoses and control the treatment of benign and malignant diseases, immunodeficiencies, and Paroxysmal Nocturnal Hemoglobinuria (PNH).

Important indicators of Flow Cytometry department are:

  • Establishing a quality management system in the framework of national and ISO standards (ISO 9001 + 9001)
  • Use 10-color flow cytometry to increase sensitivity and detection characteristics
  • Use of International Standard Guidelines for Immunophenotyping for Acute and Chronic Leukemia
  • The first center in Iran to assess the minimum remaining disease (MRD) in patients under treatment for acute and chronic cancers of the blood.
  • Applying the standard high sensitivity method for diagnosis of anemia due to Paroxysmal Nocturnal Hemoglobinuria (PNH)
  • Evaluation and counting of lymphocytic subgroups in order to identify and categorize immunodeficiency diseases, infertility and …
  • Evaluation of the involvement of body fluids including cerebrospinal fluid, pleura and ascites with malignant blood and non-blood cells, in accordance with international standards.
  • Preparing results in less than 24 hours and in emergency situations between 2-4 hours
  • Having the necessary experience and knowledge and getting scientific advice from university professors in time of need

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